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Living Modified Organism
(LMO)
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Chicory modified for increased inulin expression
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Not available
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Organization:Plant Research International (), Dept. Genetics and BreedingPhone:Fax:Email:Website:
The aim of the genetic modification is to reinforce the SST activity naturally present in the chicory root. Besides this main trait the transformed chicory also expresses the nptII gene resulting in resistance to the antibiotic kanamycin.
It is expected that this extra SST will compensate for the decrease of activity of the native SST. This compensation should result in the maintenance, or even increase, of the mean degree of polymerisation of the inulin in the transgenic chicory roots. After a frost period fructan exohydrolase (FEH)is expressed in chicory’s roots. FEH catalyses the depolymerization of fructan, decreasing the value of the extracted fructan at harvest. It is expected that the extra SST introduced in the GM chicory will contribute to the re-synthesis of fructan after a frost period.
EN
It is expected that this extra SST will compensate for the decrease of activity of the native SST. This compensation should result in the maintenance, or even increase, of the mean degree of polymerisation of the inulin in the transgenic chicory roots. After a frost period fructan exohydrolase (FEH)is expressed in chicory’s roots. FEH catalyses the depolymerization of fructan, decreasing the value of the extracted fructan at harvest. It is expected that the extra SST introduced in the GM chicory will contribute to the re-synthesis of fructan after a frost period.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12086-3 Organism Cichorium intybus (Chicory)Crops
Cultivar/ Breeding line: 95/9 and 145/1
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pSST331 and pA33236 (derived from pBINPLUS)
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-46095-2 Sucrose:sucrose 1-fructosyl transferase gene | Helianthus tuberosus (Sunflower, Jerusalem artichokes , Sunroot, Sunchoke)Protein coding sequence | Increased inulin
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103886-2 5' Untranslated Leader of AMV RNA4 | Alfalfa mosaic virus (Alfalfa mosaic virus, AMV)Leader
Inserted DNA
Two vectors were constructed for transformation each with a diffrent version of the Sucrose, sucrose fructosyltransferase gene as indicated:
T-DNA from pSST331 - sst103: Sucrose, sucrose fructosyltransferase from Helianthus tuberosus;
T-DNA from pA33236: - a33: Sucrose, sucrose fructosyltransferase from Helianthus tuberosus;
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Two vectors were constructed for transformation each with a diffrent version of the Sucrose, sucrose fructosyltransferase gene as indicated:
T-DNA from pSST331 - sst103: Sucrose, sucrose fructosyltransferase from Helianthus tuberosus;
T-DNA from pA33236: - a33: Sucrose, sucrose fructosyltransferase from Helianthus tuberosus;
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- Food
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EN
- Notification Report - European Commision [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
